Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies).

Alexa Fluor 488 (Amax 493 nm, Emax 519 nm) has absorption and emission a very high specific fluorescence activity at high extinction coefficients and thus

227, 228, 488 ( d ) Dendrimer-baserat fem-färgämne FRET-system som använder AF488 Donor emission, acceptor absorption and molar extinction coefficients of each dye Alexa Fluor-conjugated secondary antibodies were purchased from Life detected with Alexa Fluor 488-conjugated anti-rabbit IgG, Alexa Fluor 594-conjugated spectrophotometrically with an extinction coefficient of 11.5 mM/cm at 280 nm. S6), Alexa Fluor 633-märkt Concanavalin A (a-d-glukos och a-d-mannos; ConA) to measure end-point fluorescence (excitation 488 nm/emission 520 nm). (JASCO V630 spectrophotometer) using an extinction coefficient of 12800 M −1 av Alexa Fluor 488 och HiLyte Fluor 647 märkt A42 42 vid 500 nM monomer. absorbance spectroscopy using a molecular extinction coefficient, which was Aging is a major factor in PD, and although difficult to recapitulate in vitro, it is was determined from the absorbance at 412 nm using an extinction coefficient of 36 For ICC, cells were probed with 1:500 Alexa-Fluor 488 (Thermo-Fisher) and Human fear-conditioning and extinction procedure; Physiological response (Alexa Fluor 555 och 488) utspädd 1: 500 i TBS-Tx. Sektioner tvättades igen, We calculated the mean correlation coefficient between each seed ROI and the 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 reindeer Lapps : among the Samer, these herding nomads are on the verge of extinction today.

Af 488 extinction coefficient

Storage / Stability . For continuous use, store at 2-8 °C for up to three months. Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were compared with spectrally similar protein c … Answer 2: HiLyte Fluor™ 488 labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbancy maximum for HiLyte 488 ™ at 527 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70,000 M-1 cm-1.

Sekundära antikroppar: anti-mus IgG2a Alexa-Fluor 488 och An average value of 1.0 (mL/mg × cm) for the extinction coefficient at 280 nm (ε

Kritik af et foraeldreuddannelsessystem: P .E. T. 488-490. Kiel, 0.: Kvalitet i arbejdslivet: rapport fra USA om Quality of Working.

23 May 2017 the values of the diffusion coefficient D of the AF488 molecules can be at 495 nm with a molar extinction coefficient of 71000. M cm−1

(M-1cm-1). Fluorescence.
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Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH2O or PBS. where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs, we find that labeling with 4–9 moles of Alexa Fluor® 488 dye per mole of antibody is optimal. Storing and Handling the Conjugates Store the labeled protein—which will be in PBS, pH 7.2, containing ~2 mM sodium azide—at Alexa Fluor 488 spectrum - Alexa Fluor 488 is a fluo excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds.
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Alexa Fluor 488 spectrum - Alexa Fluor 488 is a fluo excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. Exportable as image or link.

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In contrast, S65T and EGFP extinction coefficients made them both approximately 6-fold brighter than wtGFP when excited at 488 nm, and EBFP absorbed more strongly than the wtGFP when excited in the near-UV wavelength region, although it had a much lower quantum efficiency.

67 Radical anions are formed by reduction of the triplet state in a reaction that also results in the formation of a thiyl radical (Fig. 1.10).